%0 Journal Article
%T Establishment and Optimization of the Regeneration System for Common Dandelion (Taraxacum officinale Weber)<br>药蒲公英再生体系的建立和优化
%A Hua Chen
%A Ping Li
%A Jing Liu
%A Yin-Xin Li
%A <br>陈华
%A 李平
%A 刘晶
%A 李银心
%J 生物工程学报
%D 2005
%I 
%X A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Mult iple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For i ndirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerate d pl antlets were obtained when these calli were cultured on MS medium containing 1. 0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific b ands.
%K common dandelion
%K callus culture
%K organogenesis
%K R APD<br>药蒲公英
%K 愈伤组织培养
%K 器官发生
%K RAPD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6C45F296BFB61C2F&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=0B39A22176CE99FB&sid=5B5B75F4854B8331&eid=D537C66B6404FE57&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=21