%0 Journal Article
%T Purification and Production of the Extracellular 5-aminolevulinate from Recombiniant Escherichia coli Expressing Yeast ALAS<br>表达酿酒酵母ALAS的重组大肠杆菌胞外5-氨基乙酰丙酸的产量和纯化
%A HE Xiao-Mei
%A ZHOU Jing
%A CHENG Ying
%A FAN Jun
%A <br>何晓梅
%A 周静
%A 程郢
%A 范军
%J 生物工程学报
%D 2007
%I 
%X Aminolevulinic acid (ALA) is biosynthesized by the enzyme ALA synthase (ALAS). The ALA production has been studied using the overproducing ALAS from several bacteria in Escherchia coil, respectively. However, ALAS from eucaryote expressed in E. coli for producing ALA in the culture is not known. The extracellular ALA production and cell growth were investageted respectively using the recombinant E. coli overproducing Saccharomyces cerevisiae ALAS in shake-flask fermentations. The ALAS activity from the cell extract was assayed. The extracellular ALA was purified by the national-made large-dimension resins and confirmed by the capillary electrophoresis measurements. At 12h after induction at 37 degrees C, the extracellular ALA production was up to 162mg per liter LB culture at initial pH 6.5 with exogenous levulinate, succinate and and glycine at the concentration of 20 mmol/L respectively. The purity of ALA after purification is up to 90%.
%K hem1<br>酿酒酵母
%K 5-氨基乙酰丙酸产量
%K 5-氨基乙酰丙酸纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FDBF5E592DDB387E&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=F3A3627BFEF439C1&eid=500A64A3035F6B9C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18