%0 Journal Article
%T The Construction and Expression of Superantigen SEA and Antimelanoma ScFv Fusion Gene<br>超抗原SEA与抗黑色素瘤ScFv融合基因的构建及表达
%A Jing Sun
%A An-Guo Lü
%A Wen-Fang Wu
%A Xiang-Yang Bai
%A Xiu-Bao Ren
%A Hong Liu
%A <br>孙静
%A 吕安国
%A 吴文芳
%A 白向阳
%A 任秀宝
%A 刘虹
%J 生物工程学报
%D 2003
%I 
%X Two strategies, direct ligation after enzyme digestion and over-lap PCR technology, were adopted to construct a fusion gene which was composed of the antimelanoma single chain antibody gene and the staphylococcal enterotoxin A gene without N-terminal signal sequence. The fusion gene was subcloned into pET28-a vector and transformed into E. coli BL21(DE3). Ni-NTA system was selected to separate and purify the expresstd products. The inhibition ratio of the fusion protein was tested by MTT method. It is shown that the 6His-ScFv-SEA fusion protein can be expressed stably in E. coli BL21 (DE3). The quantity of the fusion protein was shown up to 30% of the total protein of the bacteria and mainly in inclusion body. By activation the effective cells,the fution protein can inhibit the melanoma cell whith expressed corresponding antigen.
%K antimelanoma single chain antibody
%K staphylococcal enterotoxin A
%K fusion protein
%K target therapy<br>抗黑色素瘤单链抗体，金黄色葡萄球菌肠毒素A，融合蛋白
%K 靶向治疗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B62461310F7A0750&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=626A0FE8E3130AB5&eid=04CF423CEE45E258&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=7