%0 Journal Article
%T Cloning and Sequence Analysis of the Gene Encoding NiFe-hydrogenase from Klebsiella pneumoniae<br>克氏肺炎杆菌NiFe-氢酶基因的克隆与序列分析
%A LIU Fei
%A FANG Bai-Shan
%A <br>刘飞
%A 方柏山
%J 生物工程学报
%D 2007
%I 
%X Degenerate PCR primers were designed by multiple alignment of the protein sequences of known structural genes encoding the catalytic subunits of NiFe-hydrogenases obtained from Swiss-Prot Protein Sequence Database through CLUSTAL-W software and compared for conserved sequence motifs. An amplified PCR product 1 kb in size was obtained from the genomic DNA of Klebsiella pneumoniae using a set of degenerate primers, and then inverse PCR technique was used to obtain the full hydrogenase coding region. A predicted secondary structure and 3D structural model were constructed by homology modeling and docking. On the basis of these results, it was inferred that NiFe-hydrogenase from Klebsiella pneumoniae belongs to the membrane-bound H_2 evolving hydrogenase group (Ech hydrogenase group).
%K Klebsiella pneumoniae
%K NiFe-hydrogenase
%K sequence analysis
%K structure prediction
%K docking<br>克氏肺炎杆菌
%K NiFe-氢酶
%K 序列分析
%K 结构预测
%K 分子对接
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=BE2B384ADF5A001F&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=76B5E24D6EC46B4B&eid=205BE674D84A456D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=21