%0 Journal Article
%T Gene Expression and Activities Analysis of a New Fusion Protein (RGD)3/tTF<br>一种新型融合蛋白(RGD)3/tTF的基因表达与活性分析
%A YAN Jiang-Hua
%A YANG Gui-Wang
%A WANG Jie-Ping
%A WU Na
%A ZHUANG Guo-Hong
%A <br>颜江华
%A 杨桂旺
%A 王阶平
%A 吴娜
%A 庄国洪
%J 生物工程学报
%D 2007
%I 
%X To develop a new fusion protein (RGD)3/tTF for the therapy of the selective thrombosis of tumor blood vessels. The fused gene (RGD) 3/tTF was reconstructed by PCR, was cloned into vector pET22 b(+), and expressed in E. coli BL21 (DE3). The fusion protein was purified through Nickel-affinity chromatography column. The tTF activity of the fusion protein was detected by clotting assay and F X activation assay. The specific binding of (RGD) 3/tTF to alphavbeta3 was analyzed by indirect ELISA. The recombinant plasmid pET22 b(+)/(RGD)3/tTF was obtained and expressed in E. coli BL21 (DE3). The purified fusion protein could induce blood coagulation, activiate F X. The ability of (RGD) 3/tTF binding specifically to alphavbeta3 was increased by 32%, compared with RGD/tTF. A new fusion protein (RGD) 3/tTF was successfully expressed in E. coli BL21 (DE3). The expressed proteins retained tTF activity and showed a higher binding to alphavbeta3 than that of RGD/tTF.
%K RGD<br>关键词tTF
%K 融合蛋白
%K 凝血
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9417E2437EA101D5&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=38B194292C032A66&sid=0493D643315CD829&eid=216EFB25F7F834CC&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=7