%0 Journal Article
%T The Preparation of Fructose-modified Chitosan Microcarrier and Culture of Primary Rat Hepatocyte<br>果糖修饰壳聚糖微载体的制备及其原代大鼠肝细胞培养
%A ZHANG Li Guo PAN Ji Lun
%A LI Jie Liang YU Yao Ting
%A <br>张立国
%A 潘继伦
%A 李结良
%A 俞耀庭
%J 生物工程学报
%D 2003
%I 
%X Crosslinked chitosan microcarries were prepared by the reaction of glutaraldehyde with fructose-modified chitosan. Various factors that influence the preparation were studied and the reaction conditions were optimized. Morphology of rat hepatocyte cultured on chitosan microcarriers was observed using phase contrast microscopy and scanning electron microscope, the metabolic activity was measured. Rat hepatocytes cultured on chitosan microcarrier retained the spherical shape as they have in vivo and had high metabolic activity. Fructose can enhance the metabolic activity of hepatocytes and fructose-modified chitosan microcarrier is a promising scaffold for hepatocytes attachment, which can be used in bioartificial liver support system.
%K fructose
%K chitosan
%K microcarrier
%K hepatocyte culture<br>果糖，壳聚糖，微载体，肝细胞培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2DCFDCF4584EDEF6&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=8477411EEDB08A86&eid=EFD65B51496FB200&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=12