%0 Journal Article
%T The Expression of Humanized Fab Fragment of the Anti-HBsAg Antibody in Methylotropic Yeast Pichia pastoris<br>人源性抗HBsAg抗体Fab段在酵母中的表达
%A DENG Ning
%A SU Kuan-Yuan
%A WANG Xun-Zhang
%A LONG Qing-Xin
%A YANG Lin
%A YU Zhou-Yao
%A <br>邓宁
%A 粟宽源
%A 王珣章
%A 龙綮新
%A 杨林
%A 余宙耀
%J 生物工程学报
%D 2002
%I 
%X Using of two-step integrating technology, transducted the H and L chain gene of humanized Fab fragment of anti-HB-sAg antibody into the genome of methylotropic yeast P. pastoris. Constructed a engineering yeast to produce humanized Fab fragment of the anti-HBsAg antibody. The Fab fragment was efficiently secreted into the medium at a concentration of 50-80 mg/L. The Fab fragment was purified from culturing supernatant of the recombinant yeas by affinity chromatography. The ELISA analysis showed the high affinity of the expressed humanized Fab fragment to the HBsAg.
%K humanized Fab
%K secreting expression
%K affinity purification
%K Pichia pastoris<br>人源性Fab，
%K 分泌表达，
%K 亲和纯化，
%K 巴斯德毕赤酵母
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0CD426B8DAFF4AC5&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=DEBDB7F30FBA7F9B&eid=64808317C39DF331&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=13&reference_num=12