%0 Journal Article
%T Construction of Gene Library of Arthrobacter BT801 and Isolation & Expression of Hydantoinase Gene<br>节杆菌BT801基因文库构建及其乙内酰脲酶基因分离与表达
%A HAO Shu Feng
%A ZHANG Wei Cai YUAN Hong Jie WANG Heng Liang HUANG Liu Yu
%A <br>郝淑凤
%A 张惟材
%A 袁红杰
%A 王恒梁
%A 黄留玉
%J 生物工程学报
%D 2003
%I 
%X Hydantoinase can be widely used in enzymic production of various amino acids. In order to obtain the hydantoinase genes in Arthrobacter BT801, its chromatosomal DNA is isolated and partialy digested with Sau3A I to collect fragments of about 30kb. Then, this fragment is inserted into the Hpa I and Pst I site of cosmid pKC505. The genomic library was thus constructed by packing in vitro with lambda phage package protein and transfecting E. coli DH5alpha. A positive transformant was selected from the library using thin layer chromatography and other methods. A DNA fragment containing complete hydantoinase genes was sequenced by sub-cloning into pUC18. The gene can express active protein under control of its own promoter and T5 promoter in E. coli. The isolation of the gene established foundition for research and application of the hydantoinase.
%K hydantoinase
%K Arthrobacter
%K gene library
%K gene expression<br>乙内酰脲酶，
%K 节杆菌，
%K 基因文库，
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=1EE98994B1C1148D&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=C1B34927D429E92F&eid=B8F8200D88DDC7D6&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=7&reference_num=11