%0 Journal Article
%T Construction of Eukaryofic Expression Vector Using Neomycin-resistance Gene Mutant as Selectable Marker<br>以新霉素抗性基因突变体为筛选标志的真核表达载体的构建
%A GAO Chuan
%A ZHU Xu-Dong ZHOU Xiao-Wei YU Fang LU Bai-Song HUANG Pei-Tang
%A <br>高川
%A 朱旭东
%A 周晓巍
%A 于芳
%A 卢柏松
%A 黄培堂
%J 生物工程学报
%D 2002
%I 
%X Neomycin-resistance gene is widely used as a selectable marker in eukaryotic expression vector. It codes neomycin phosphotransferase II (NPT II) which confers resistance to various aminoglycoside antibiotic such as G418 and kanamycine. In this work, by site-directed mutagenesis the neo gene mutant was obtained. The expression vector pmDNA using the neo gene mutant as selectable marker has been constructed. After inserting interest luciferase gene, the expression plasmid pmDNAluc + was stably transfected CHO-K1 cells. As a result, the expression positive ratio reaches to approximate 95% and the ratio of high expression colonies is apparently higher than the controls.
%K eukaryotic expression vector
%K mutant
%K selectable marker gene<br>真核表达载体，
%K 突变体，
%K 筛选标志基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=484D0182F9E03669&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=51C74DF6A16DA45B&eid=7737D2F848706113&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=11