%0 Journal Article
%T Expression of Adenovirus-mediated pGH cDNA with First Intron in CHO Cells<br>腺病毒介导的含第一内含子pGH cDNA在CHO细胞中的表达
%A LI Xiu
%A |Jin ZHONG Fei WANG Xin
%A |Zhong QI Shun
%A |Zhang
%A <br>李秀锦
%A 仲飞
%A 王辛中
%A 齐顺章
%J 生物工程学报
%D 2002
%I 
%X The recombinant adenoviruses containing pGH cDNA and pGH cDNA with the first intron under the control of CMV promoter were constructed respectively by homogenous recombination method.The results showed that the recombinant adenovi ruses could mediate pGH cDNA expression in CHO cells infected with the recombinant adenoviruses.The expression level of pGH cDNA with the first intron increased by 117% compared with pGH cDNA without intron.This indicate that the first intron of pGH gene have the function of improving the expression of the pGH gene.
%K porcince growth hormone
%K first intron
%K adenovirus vector<br>猪生长激素基因
%K 第一内含子
%K 腺病毒载体
%K CHO细胞
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B4F84559617F0A0A&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=0B39A22176CE99FB&sid=23104246A5FCFCEF&eid=2BA123C6EB9D54C2&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=16