%0 Journal Article
%T Study on the Culture of Crown Gall from Panax quinquefolium and the Prouction of Its Secondary Metabolites--Ginsenosides Re and Rg1<br>西洋参冠瘿组织培养及其人参皂苷Re和人参皂苷Rg1的产生
%A YU Rong Min
%A SONG Yong Bo
%A ZHANG Hui
%A YE Wen Cai
%A ZHANG Yin Lin
%A YAO Xin Sheng
%A <br>于荣敏
%A 宋永波
%A 张辉
%A 叶文才
%A 张荫麟
%A 姚新生
%J 生物工程学报
%D 2003
%I 
%X It was clearly demonstrated that T-DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown gall in our experiment. This paper reported the influences of some kinds of physical-chemistry factors on the growth of crown gall of Panax quinquefolium and the production of its main active compounds--ginsenoside Re and ginsensoside Rg1. The results showed that White medium was the best one for ginsensoside Rg1 accumulation (0.095%) among the six media, but ginsensoside Re accumulation (0.194%) was the highest on the MS medium; The highest contents of ginsensoside Re (0.147%) and ginsensoside Rg1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5.6 for ginsensoside Rg1 synthesis(0.054%), and 5.8 for ginsensoside Re synthesis(0. 184% ); The contents of ginsensoside Re and ginsensoside Rg1 was the highest in the inoculum of 4 g and 2 g/flask (FW) respectively. The result also indicated that the concentration of inositol in 0.05 g/L could obviously promote ginsensoside Re synthesis (0.182%), and in 0.30 g/L for ginsensoside Rg1 (0.055%).
%K Panax quinquefolium
%K crown gall culture
%K ginsenoside Re
%K ginsenoside Rg
%K 1
%K HPLC<br>西洋参
%K 冠瘿组织培养
%K 人参皂苷Re
%K 人参皂苷Rg1
%K 高压液相色谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D5E7D386D5E35BAD&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=965F4E89CD0AFC30&eid=315A3D008C6ECFC8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=11&reference_num=5