%0 Journal Article
%T Purification and Characterization of Antifungal Proteins in Triticale Seed<br>小黑麦抗真菌蛋白组分的分离纯化和性质研究
%A NA Bing
%A YU Ming-Kun
%A GONG Jun
%A WU Jin
%A <br>那冰
%A 余明琨
%A 龚隽
%A 吴襟
%J 生物工程学报
%D 2002
%I 
%X Using Trichoderma as an indicative fungus, three antifungal proteins in Triticale Zhongsi 237 seed were purified and characterized. These protein components were considered to be a new Class II chitinase and two kinds of beta-1, 3-glucanases. Chitinase molecular mass was 30.5 kD and enzyme activity was maximal at pH 6.0 and 37 degrees C. Two beta-glucanases molecular masses were 51 kD and 23 kD. N-terminal amino acid sequences of Triticale chitinase share high homology with barley chitinase. In some conditions, the chitinase and beta-glucanases all had strong antifungal activity and were able to inhibit Trichoderma growth synergistically. Moreover, the chitinase and beta-1, 3-glucanases were able to inhibit powdery mildew growth on detached susceptible wheat leaves.
%K 小黑麦，
%K 抗真菌蛋白，
%K 分离纯化，
%K 几丁质酶，
%K β-1.3-葡聚糖酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=23879753256BBE01&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=B4F9D541F855CF96&eid=90075EB19043D533&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=12