%0 Journal Article
%T Cloning and Expression of MGMT cDNA and Analysis of the DNA Repair Activity of the Recombinant Protein<br>人MGMT基因Cdna的克隆表达及表达蛋白修复功能的鉴定
%A ZHU Yu-Wen
%A LIU Jian-Guo
%A LI Gao-Xiang
%A <br>朱玉文
%A 刘建国
%A 黎高翔
%J 生物工程学报
%D 2001
%I 
%X A cDNA encoding human O6-methylguanine-DNA methyltranferase (MGMT) was cloned from Hela S3 cells and the sequence is identical with the published data.The MGMT cDNA was inserted into the expression vector pET-21a and transformed into \%E.coli\% BL21 (DE3).A 24 kD protein was expressed after IPTG induction.Essays using lethal dose of alkylating agents indicate that expression of MGMT protein can repair the DNA mutations of the recombinant bacteria produced by alkylating agents.
%K O6-methylguanine-DNA methyltransferase(MGMT)
%K alkylating agents DNA repair
%K gene cloning and expression<br>O6-甲基鸟嘌呤-DNA-甲基转移酶
%K 烷化剂
%K 基因克隆表达
%K DNA分子修复
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FC92360B92F8D39A&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=7D6CD8918B045FD4&eid=87545994EC2C1F12&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14