%0 Journal Article
%T The Changes of Rare Cordons Increase Expression Level of SEA<br>改造稀有密码子提高SEA蛋白表达量
%A SHI Cheng-Bo
%A LU An-Guo
%A WU Wen-Fang
%A YANG Li-Quan
%A FENG Jia-Xun
%A BAI Xue-Liang
%A <br>时成波
%A 吕安国
%A 吴文芳
%A 杨立泉
%A 冯家勋
%A 柏学亮
%J 生物工程学报
%D 2002
%I 
%X The rare codons of a fragment in staphylococcal enterotoxin A gene were turned into the most high usage frequency codons in E.coli by overlap PCR technique. Genes of sea and sea m were cloned into 7ZTS expression vector and transformed into JM109(DE3),respectively. The result shows that expression level of sea gene was very low, but the expression level of sea m was as high as 15% of total cell proteins. The expression product shows activity of antitumor in vivo .
%K expression vector
%K overlap PCR
%K gene mutation
%K rare codons<br>稀有密码子，
%K 基因突变，
%K 重叠PCR，
%K 表达载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D4A8D651FC77B396&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=F18BA6286A889C1C&eid=03436AC72A659ACA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=14&reference_num=13