%0 Journal Article
%T The Study on the Stable Inheritance and Expression of Foreign Gene in Transgenic Mice<br>转基因小鼠中外源基因遗传及表达稳定性的研究
%A YAN Jing-Bin
%A XIAO Yan-Ping FANG Yu-Dan XI Ying HUANG Wen-Ying HUANG Ying
%A <br>颜景斌
%A 肖艳萍
%A 方彧聃
%A 奚鹰
%A 黄文英
%A 黄英
%J 生物工程学报
%D 2002
%I 
%X Two transgenic mouse strains, in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32 +/- 5.41) microgram/mL in FIX-33 transgenic strain and (1.16 +/- 0.45) microgram/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P < 0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.
%K transgenic mice
%K foreign gene
%K inheritance
%K expression<br>转基因小鼠，
%K 外源基因，
%K 遗传，
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F83FD0C19F985EC1&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=3D8AB54CA690066A&eid=2A4D3806B72AF9C3C572DC2C501BD025&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=11