%0 Journal Article
%T Advances in in vitro Refolding of Inclusion Body Proteins<br>包涵体蛋白体外复性的研究进展
%A FANG Min
%A HUANG Hua
%A |Liang
%A <br>方敏
%A 黄华
%J 生物工程学报
%D 2001
%I 
%X Overexpression of recombinant proteins in \%E.coli\% often results in formation of insoluble, inactive inclusion bodies. These inclusion bodies, which contain the recombinant proteins in a highly enriched form, can be isolated by solid/liquid separation. After solubilization, active proteins can be generated through an appropriate refolding process. Within the last decade, specific strategies and methods have been developed for preparing active recombinant proteins from inclusion bodies. Recent developments in renaturation procedure include the inhibition of aggregation during refolding by the application of low molecular weight additives and matrix\|bound renaturation techniques.
%K recombination protein
%K inclusion body
%K refolding
%K renaturation
%K disulfide bond formation<br>重组蛋白
%K 包涵体
%K 重折叠
%K 复性
%K 二硫键形成
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=AB6D041EA2FDC84F&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=C7B13290323C226E&eid=EF9E84B2DA79FF23&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=35&reference_num=41