%0 Journal Article
%T AN ALTERNATIVE PLOTTING METHOD USING PROGRESS CURVES OF ENZYME REACTION TO DETERMINE KINETIC PARAMETERS<br>利用酶催化反应过程曲线确定动力学参数的新方法
%A 王志新
%J 生物物理学报
%D 1990
%I 
%X A plotting method has been developed to determine kinetic parmeters by using progress curves of enzyme reaction. The method has two are antages: 1. All kinetic constants, Km, Vmax , Kp and Kp, can be obtained in only two experiments; 2. All plots are linear which may provide a more reliable estimation for the reaction mechanisms and the kinetic constants. In addition, this method is also applicable to the cases of substrate inhibition and simple two - substrate. enzyme reaction, and hence it may be useful in study on the kinetics of enzyme - catalyzed reaction.
%K 酶催化反应
%K 过程曲线
%K 动力学参数
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=1FB8677628C2078C3B948A04B3CEA8F9&yid=8D39DA2CB9F38FD0&vid=B31275AF3241DB2D&iid=0B39A22176CE99FB&sid=6CCE24D86D03D083&eid=6A73B36E85DB0CE9&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0