%0 Journal Article
%T Fusion Expression of Human Thymosin α1 in Escherichia coli<br>人胸腺素α1在大肠杆菌中的融合表达
%A XIU Zhao-Yang
%A YU Ying
%A CHEN Chang-Qing
%A <br>修朝阳
%A 周穗菁
%A 俞璎
%A 陈常庆
%J 生物工程学报
%D 2002
%I 
%X Engineering E. coli strain, BL21 (DE3)/pGEX-4T-human Thymosin alpha 1, was constructed by oligonucleotide annealing and PCR amplifying the target gene, then ligating it with pGEX-4T-3 vector and transferring into BL21 host. The yield of fusion protein of GST-Thymosin alpha 1 expressed from BL21 (DE3)/pGEX-4T-thymosin alpha 1 is about 35%-40% of total protein after fermentation. Following the simple cut of thrombin or CNBr, about 0.2 g/L thymosin alpha 1 can be harvested. The product is checked by MS and activity test, which indicates that the recombinant product has full biological activity of native thymosin alpha 1.
%K GST<br>溴化氰降解，
%K 凝血酶加工，
%K 淋巴细胞测活，
%K GST
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=285CC966F81C11CE&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=1918ADDC93A85779&eid=6313C162FF75889A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=11