%0 Journal Article
%T Study of the Expression Membrane Protein LASS2<br>膜蛋白LASS2的表达研究
%A CAI Xing
%A Feng
%A TAO Zhen
%A CAO Yu
%A YANG Sheng
%A Li
%A GONG Yi
%A <br>蔡兴锋
%A 陶震
%A 曹郁
%A 杨胜利
%A 龚毅
%J 生物工程学报
%D 2003
%I 
%X A human membrane protein LASS2 ( Homo sapiens longevity assurance homologue 2 of yeast LAG1 ), which has important physiologic functions, was expressed in three different expression systems. Only the LASS2 protein carboxyl terminal hydrophilic fragment could be expressed in the prokaryote expression system and its polyclonal antibody was produced. The full length of LASS2 protein could be expressed successfully in both eukaryotic in vitro translation system and Bacuvirus expression system:Bac to Bac system. SDS PAGE analysis revealed that the molecular weight of expressed product of LASS2 was about 28 kD. The product was also proved by Western blot. This recombinant LASS2 protein was purified by metal affinity resin and the purity is above 90%.
%K membrane protein
%K bacuvirus expression system
%K eukaryotic
%K in vitro
%K translation system
%K affinity chromatography<br>膜蛋白
%K 昆虫表达系统
%K 真核体外翻译系统
%K 亲和层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=24E250E55974D3B7&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=CB423C9A71560A74&eid=B9704B40A4225A24&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=8