%0 Journal Article
%T DIGITALIZATION IN ULTRA-SENSITIVE FLUOPESENCE MICROSCOPY AND ITS APPLICATION IN PHOTO-BIOLOGY RESEARCH<br>高灵敏度荧光显微镜量化技术及其在光生物学研究中的应用
%A Chen Tianming
%A Wang Susheng
%A Chen Tianming
%A Sun Jiangzheng
%A <br>陈天明
%A 王苏生
%A 俞信
%A 孙江政
%A 曹恩华
%A 许娜飞
%A 李景福
%J 生物物理学报
%D 1995
%I 
%X An ultra-sensitive fluorescence microsocopy has been developed with the image intensifier as its detector. Its sensitivity is 4 ×104 higher than that of ordinary fluorescence microscopy, and the linear coefficiency between light intensity and CCD output was obtained by using a wide-range ultra-weak photometer. So the instrument can not only show the image of ultra-weak fluoresrmce distribution, but also give the intensities of fluorescence of each iTnage panel as well as that of a Cell. Thus, the instrument can be used to detect trivial changing of light intenSity or light distribution which is toossible to be obserVed with human eye directly.Using this ulha-sensitive fluorescence microscopy, we have got the image of Hypocrellin A distribution in Hela Cells and its three dimensional display, we have also got the cage of Hela Cell being oxidated after exposed to strong light.
%K Fluorescence microscopy Digitalization Hypocrellin A<br>光生物学
%K 荧光显微镜
%K 量化技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=8760C2861E15B2FE940E6D7FDA27DFE9&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=38B194292C032A66&sid=BD7D27247C63490C&eid=0A0BD3F594F876C0&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=6