%0 Journal Article
%T High Expression and Characterization of Human Parathyroid Hormone in Escherichia coli<br>人甲状旁腺激素在大肠杆菌中的表达及鉴定
%A Hong-Qing Fang
%A Hong-Mei Dai
%A Yan-Ying Li
%A Hong-Liang Zhao
%A Bing-Bing Deng
%A Chong Xue
%A Zhi-Min Liu
%A Hou-Chu Zhu
%A Qing-Jun Ma
%A Hui-Peng Chen
%A <br>方宏清
%A 戴红梅
%A 李彦英
%A 赵洪亮
%A 刘志敏
%A 朱厚础
%A 马清钧
%A 陈惠鹏
%J 生物工程学报
%D 2003
%I 
%X Human parathyroid hormone(hPTH) was highly expressed in Escherichia coli by inserted the synthesized whole hPTH cDNA into the vectors pBV220 and pET22b.After expression and disruption,the purified product was acquired through cation exchange chromatography and reverse phase chromatography.From the results of N terminal sequencing and MALDI TOF MS analysis the recombiant prtein was indentified as intact hPTH.In in vitro Bioassays the recombinant hPTH stimulated adenylate cyclase as the standard did. In ovariectomized rats the recombinant hPTH markedly increased the femoral bone mass and bone mineral density.
%K human parathyroid hormone
%K Escherichia coli
%K gene expression<br>人甲状旁腺激素，
%K 大肠杆菌，
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CCBE0F5531165350&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=331211A5F5616413&eid=F24949CFDB502409&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=6