%0 Journal Article
%T Enzymatic Synthesis of Monoglycerides in Microaqueous Media by Using Lipase from Pseudomonus fluorescens<br>微水体系中荧光假单胞菌脂肪酶催化合成单甘酯
%A XIA Yong-Mei
%A ZHANG Ke-Chang
%A SHI Gui-Yang
%A LI Xiao-Hui
%A FANG Yun
%A <br>夏咏梅
%A 章克昌
%A 石贵阳
%A 李晓晖
%A 方云
%J 生物工程学报
%D 2002
%I 
%X Enzymatic synthesis of monoglycerides by glycerolysis of oil and fats in microaqueous solvent-free media was investigated by using lipase from pseudomonus fluorescens (PFL). Initial eutectic point(IEP) was substituted for melt point of oil and fats in Critical Temperature Theory. By investigating the glycerolysis under different IEP, it is showed that there is a relationship between composition of the oils and the yield of monoglycerides: Y = -0.0006 X3 + 0.0592 X2 - 0.8909 X + 26.753(13% < X < 76.5%), here X is the contents(W/W) of saturated fatty acid residue (C16 + C18) in the oils, Y is the yield of monoglycerides at 40 degrees C. The optimum isothermal reaction conditions for a system which IEP is 40 degrees C are: 40 degrees C, 3%-4.5% (W/W) water in glycerol, dosage of lipase is 500 u/g oil when the mole ratio of glycerol to oil is 2.5:1. The highest yield of monoglycerides is 81.4% in 48 h by means of programming temperature reaction.
%K microaqueous media
%K lipase
%K monoglycerides
%K eutectic point
%K specificity<br>微水体系
%K 脂肪酶
%K 单甘酯
%K 最低共熔点
%K 基质特异性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B8A247D83081594A&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=656F8C8401D91023&eid=7E8E8B150580E4AB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=12