%0 Journal Article
%T THE EFFECT OF EMODIN ON THE INCREASE OF [Ca2+]i AND TNF-α PRODUCTION IN LIPOLYSACCHARIDE-STIMULATED RAT MACROPHAGES<br>大黄素影响巨噬细胞升高[Ca2+]i 和释放TNF-α的作用特征
%A 王辉
%A 董志勇
%A 余奕
%A 刘保华
%A 马涛
%A 简序
%J 生物物理学报
%D 2002
%I 
%X To explore the role of emodin on increase in Ca2+]i and TNF-α production in normal and lipopolysaccharide (LPS)-stimulated rat peritoneal macrophages (PMφ), TNF-α production was determined using TNF-α-sensitive L929 cell line and by MTT method and the kinetics of intracellular free Ca2+ concentration (Ca2+]i) in a single cell were monitored continuously by confocal laser scanning microscope. The results showed that 10-5 mol/L emodin could elevate TNF-α production slightly, and caused forming low amplitude Ca2+]ioscillation in normal single PMφ. Emodin could inhibit both excessive release of TNF-α and increase in Ca2+]i when PMφ was stimulated by LPS. 10-5 mol/L emodin inhibited TNF-α production by 50% and Ca2+]i increase by 68% respectively. Interestingly, emodin could transform the high amplitude "plateau-like" Ca2+]i induced by LPS into fluctuation that has low amplitude. These results indicate that the biphase characteristics of modulation of Emodin on TNF-α production correlate with Ca2+]i changing. The modulation of emodin on Ca2+]i may be a main way that emodin inhibits TNF-α production in LPS stimulated PMφ.
%K 大黄素
%K 巨噬细胞
%K TNF－α
%K 作用特征
%K 脂多糖
%K 细胞内自由Ca^2＋浓度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=9809D90B8435919E&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=3622B70F9C54A9CC&eid=31125890FF093250&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=6&reference_num=15