%0 Journal Article
%T Cloned Goats Produced from the Somatic Cells of an Adult Transgenic Goat<br>由成年转基因山羊体细胞而来的克隆山羊
%A CHENG Yong
%A WANG Yu-Ge
%A LUO Jin-Ping
%A SHEN Yu
%A YANG Yue-Fei
%A JU Hui-Ming
%A ZOU Xian-Gang
%A XU Shao-Fu
%A LAO Wei-De
%A DU Miao
%A <br>成勇
%A 王玉阁
%A 罗金平
%A 沈玉
%A 杨跃飞
%A 鞠辉明
%A 邹贤刚
%A 徐少甫
%A 劳为德
%A 杜淼
%J 生物工程学报
%D 2002
%I 
%X This study was carried out to examine the effect of different donor cell type and micro-manipulation on the development of reconstituted embryos. Cultured mural cumulus cells or fibroblast cells from an adult transgenic goat expressing human erythropoietin(rhEPO) were used as the donor cells in nuclear transfer experiments. The reconstituted eggs were generated by transferring fibroblast cells or cumulus cells into the perivitelline space of enucleated M II oocytes and then followed by electrofusion and activation. After 6 days' incubation in vivo, the reconstructed embryos developed into morulae or blastocysts were transferred into 6 foster recipients. Two of the foster-mothers were pregnant and gave birth to two offspring, which were derived from the fibroblast cell and cumulus cell, respectively. Fingerprint analysis showed that the PCR-RFLP patterns of the two offspring were identical to that of donor goats. PCR results indicated that these cloned goats carried hEPO gene as same as their donor cells.
%K goat
%K transgenic animal
%K fibroblast cells
%K cumulus cells
%K cloning<br>山羊
%K 转基因动物
%K 成纤维细胞
%K 颗粒细胞
%K 克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3EFCC0F5B78543D6&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=9C65ADEB5990B252&eid=06EA2770E96C5402&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=21&reference_num=15