%0 Journal Article
%T Continuous Perfusion Culture Hybridoma Cells for Production of Monoclonal Antibody<br>连续灌流培养杂交瘤细胞生产单克隆抗体
%A MI Li
%A LI Ling
%A FENG Qiang
%A YU Xiao-Ling
%A CHEN Zhi-Nan
%A <br>米力
%A 李玲
%A 冯强
%A 余晓玲
%A 陈志南
%J 生物工程学报
%D 2002
%I 
%X Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media. The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amount of production was increased about 2-3 times. The inoculated cell density was 2.5 x 10(5) cells/mL, while the final cell density was 8.79 x 10(8) cells/mL. Antibody production was reached 295 mg/L/d at average level, and the highest level was reached 532 mg/L/d. These results provided a primary mode of enlarge culture for monoclonal antibody industralization.
%K perfusion culture
%K hybridoma
%K monoclonal antibody
%K high density<br>灌流培养，
%K 杂交瘤细胞，
%K 单克隆抗体，
%K 高密度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F73E717423640B4F&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=5DCBAAB000A70168&eid=F7496043B4F12D75&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=12