%0 Journal Article
%T Catalysis of Lyase-isomerase PecE/PecF for Seve ral Apophycobiliproteins<br>藻红蓝蛋白裂合异构酶对几种脱辅基藻胆蛋白的催化作用
%A ZHU Jing-Ping
%A ZHOU Ming
%A ZHAO Kai-Hong
%A ZENG Zhi-Xiong
%A ZHOU Yi-Kai
%A <br>朱菁萍
%A 周明
%A 赵开弘
%A 曾志雄
%A 周宜开
%J 生物工程学报
%D 2002
%I 
%X Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.
%K lyase-isomerase
%K phycobiliprotein
%K reconstitution<br>裂合异构酶，
%K 藻胆蛋白，
%K 重组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E567261CACB433C6&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=7D2B339649A57040&eid=C3BC38F6CC09E835&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=7