%0 Journal Article
%T STUDY ON THE INTERACTION OF BUFALIN WITH MEMBRANE<br>蟾毒灵与脂膜相互作用的研究
%A PAN Tie-ying
%A LUO Hui-jun
%A SHI Xin-mei
%A WANG Fu
%A LIANG Wei
%A HOU Hui-min
%A <br>潘铁英
%A 罗会俊
%A 史新梅
%A 王复
%A 梁伟
%A 侯惠民
%J 生物物理学报
%D 2000
%I 
%X Using high-resolution solid-state 13C-NMR and differential scanning calorimetry(DSC),the thermotropic and dynamic properties of bufalin in egg phosphatidylcholine(EPC)liposome model membranes were studied. The solid-state 13C-NMR, included cross polarization(CP) and magic angle spinning(MAS). DSC as a bulk method showed that bufalin lowered the main phase transition temperatrue and broadened the phase transition peak. 13C-CP/MAS experiments revealed that the chemical shift varied with temperature, indicating different degrees of trans-gauche isomerization in the lipid acyl chains when the bilayer is in the liquid crystalline phase; the specific peaks disappeared in the 13C-CP/MAS spectra when bufalin was present in preparation, indication a different mobility of the corresponding molecular moiety in phospho-lipid bilayer; the changes of relative peak intensitied also confirmed the incorporation of bufalin in the bilayer. These results suggest bufalin causes more perturbation in the lipid bilayer.
%K Bufalin
%K 13C-CP/MAS
%K DSC
%K Membrane interaction<br>蟾毒灵
%K 脂膜
%K 相互作用
%K 白血病
%K 治疗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=84B268F3ED64A5F1&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=E158A972A605785F&sid=F4C2D192FB73A21F&eid=99A964928ADB4E67&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=3