%0 Journal Article
%T STRUCTURE MODELING OF MOUSE McAb OKT3 COMBINING SITES AND DESIGN OF HUMANIZED ANTIBODY<br>鼠抗体OKT3结合位点的结构模拟和改形设计
%A Sun Jian
%A Shen Ruqun
%A Xu Lin
%A Chen Runsheng
%A <br>孙键
%A 沈如群
%A 徐琳
%A 陈润生
%A 刘喜富
%A 黄华梁
%J 生物物理学报
%D 1996
%I 
%X In order to construct reshaping anti-CD3 antibody, the combining sites of OKT3 weremodeled using the knowledge-based method. On the basis of homology of sequence, humanIg LS1 and ND were selected as acceptors for CDRs of OKT3 VL and VH. By analyzing thetertiary structure of OKT3 and comparing the primary sequence of OKT3, LS1 and ND withtheir own family sequences, some residues in the framework regions were changed and thereshaped VL and VH genes were designed. Also the structural differennces between the originalOKT3 and the reshaped antibody were analyzed. In primary experiment the ashaped antibodyshowed competitive inhibition activities against OKT3 by ELISA.
%K Structure modeling
%K Combining sites
%K Antibody Humanization<br>结构模拟，抗原结合位点，抗体，抗体人源化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=BDBED1B2AB710911AD7AC68C58D7D47C&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=EFD65B51496FB200&eid=2F56B21F91C9B05B&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=1