%0 Journal Article
%T The Construction of Shuttle Vectors of Brevibacillus brevis-Escherichia coli<br>短短小芽孢杆菌大肠杆菌穿梭分泌表达载体的构建
%A PENG Qing-Zhong
%A ZHANG Wei-Cai
%A ZHU Hou-Chu
%A <br>彭清忠
%A 张惟材
%A 朱厚础
%J 生物工程学报
%D 2002
%I 
%X The 5' region of the cell wall protein(CWP) gene containing multiple tandem promoters and the signal peptide-coding sequence was isolated by PCR from Br. brevis 50, and used to construct the shuttle vector pBKE50, which included the replication origin of pUB110 and the erythromycin-resistance gene of pGK12. The alpha-amylase gene of Bacillus subtilis 168 was ligated to pBKE50, producing plasmid pBKE50/alpha-amy. After the resulting plasmid was introduced into Br. brevis 50, soluble and biologically active alpha-amylase was secreted directly into the culture medium. The expression level of alpha-amylase in the recombinant Br. brevis 50 was twice higher than that of the donor strain.
%K Brevibacillus brevis
%K shuttle vector
%K secretory expression<br>短短小芽孢杆菌－大肠杆菌
%K 穿梭分泌表达载体
%K 构建
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6932C77D54A73A0A&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=BEE722AB5028E81F&eid=5319469C819FCFF1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=11