%0 Journal Article
%T Genomic DNA Sequences and Functional Expression,Purification of BmαTX14 Neurotoxin from Scorpion Buthus martensii Karsch<br>东亚钳蝎毒素BmαTX14的基因组克隆、真核表达纯化及功能的初步研究
%A LU Meng
%A WANG Kun
%A CAO Zhi-Jian
%A JIANG Da-He
%A MAO Xin
%A LI Wen-Xin
%A <br>吕猛
%A 王昆
%A 曹志贱
%A 蒋达和
%A 毛歆
%A 李文鑫
%J 生物工程学报
%D 2005
%I 
%X Based on the full-length cDNA of BmalphaTX14 from Chinese scorpion Buthus martensii Karsch (BmK), gene of the mature peptide of BmalphaTX14 was cloned into the yeast expression vector pPIC9K. After transforming, screening and inducing, tricine-SDS-PAGE and Western blot proved that rBmalphaTX14 protein was expressed in the medium for up to 84 hours, getting nearly 120 mg/L. Recombinant BmalphaTX14 was purified rapidly and efficiently through Ni-NTA-agarose, polyethylene glycol precipitation and gel filtration chromatography. The purified rBmalphaTX14 proved to have the anti-insect activity by toxicity assay. Meanwhile, genomic gene of BmalphaTX14 was cloned and sequenced by PCR method, sequence analysis of this gene showed that BmalphaTX14 had an intron of 408 base pairs located at the signal peptide encoding region, which was similar with the characteristic of other alpha-type sodium ion-channel toxin. Considering both the genomic organization and the peptide function, BmaTX14 proved to be a membership belonging to alpha-type sodium ion-channel toxin.
%K 蝎毒素，BmαTX14，基因组织结构，毕赤酵母，真核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3E48F462BDC32197&yid=2DD7160C83D0ACED&vid=659D3B06EBF534A7&iid=B31275AF3241DB2D&sid=970314C3B74F6C4D&eid=E002FF26604EFFAB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12