%0 Journal Article
%T Modification of Chinese Hamster Ovary Cells<br>改造中国仓鼠卵巢细胞
%A LAI Da-Zhi
%A QI Lian-Quan
%A YU Chang-Ming
%A WANG Hai-Tao
%A CHEN Wei
%A <br>来大志 齐连权 于长明 王海涛
%A 陈薇
%J 生物工程学报
%D 2002
%I 
%X Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth without limitation. So in addition to optimizing CHO system in respect of medium, environment and expression vector, modification of CHO cells themselves has drawn more and more attention. Here the main progress in CHO-modification is reviewed.
%K CHO cells
%K large-scale culture
%K apoptosis
%K cell-adhesion
%K cell cycle<br>CHO细胞
%K 改造，
%K 大规模培养，
%K 细胞凋亡，
%K 细胞黏附，
%K 细胞周期
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=48140E0D47667F20&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=BFB86B6ED3A99B9D&eid=A4E67967A1AB25F0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=41