%0 Journal Article
%T 2-Dimensional Gel Electrophoresis on Protein Patterns from Suspension Cultures of Taxus cuspidate Induced by SA<br>水杨酸作用下东北红豆杉细胞的二维凝胶电泳分析
%A QIAO Jian Jun ZHAO Hong GE Zhi Qiang YUAN Ying Jin
%A ZENG An Ping
%A <br>乔建军
%A 赵红
%A 葛志强
%A 元英进
%A 曾安平
%J 生物工程学报
%D 2003
%I 
%X The permeability of cell membrane was enhanced by exogenous SA in the culture of Taxus cuspidata Nuclei condense and fragments were observed in some cells by using fluorescent microscope, and the degraded chromosomal DNA was observed by using agarose gel electrophoresis The changes in soluble proteins of the suspension cultures of Taxus cuspidata induced by salicylic acids were analyzed by using two dimensional polyacrylamide gel electrophoresis Comparing with the control, seven new protein spots were found and six protein spots were not found in the cultures grown with SA at 48h The results obtained showed that SA could induce the expression of some special proteins that might be related with the action of SA in the suspension cultures of Taxus cuspidata
%K Salicylic acid
%K Taxus cuspidata
%K programmed cell death
%K 2
%K dimensional gel electrophoresis
%K proteome<br>水杨酸，东北红豆杉细胞，细胞凋亡，二维凝胶电泳，蛋白质组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5C07A9A3847E852C&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=08805F9252973BA4&eid=6700D0D256586E73&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=17