%0 Journal Article
%T Construction and Biological Activity Study of Human Osteoprotegerin Expressing Adenoviral System<br>人骨保护素(OPG)重组腺病毒的制备及其生物活性研究
%A LIU Ji Zhong
%A JI Zong Ling
%A HU Yun Yu
%A CHEN Su Min
%A ZHU Bang Fu
%A YANG Tong Tao
%A <br>刘继中
%A 纪宗玲
%A 胡蕴玉
%A 陈苏民
%A 朱帮福
%A 杨彤涛
%J 生物工程学报
%D 2003
%I 
%X Using the isolated total RNA from osteosacoma cell line MG63,the cDNA encoding human OPG was amplified by RT PCR. A recombinant adenoviral vector carrying cDNA of OPG was constructed and OPG expression in mouse myoblast C2C12 cells was confirmed by Western blot and ELISA The secreted expression of OPG protein persisted more than 6 weeks in vitro, and the growth of C2C12 cells infected by recombinant adenoviral were in good state Osteoclasts derived from mouse bone marrow cells infected with recombinant adenoviral made less number of TRAP positive cells and resorption pits formed on dentine slices
%K osteoprotegerin
%K recombinant adenovirus
%K osteoclast<br>骨保护素，
%K 重组腺病毒，
%K 破骨细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=BF161C9F37DA6771&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=6209D9E8050195F5&eid=1371F55DA51B6E64&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=11