%0 Journal Article
%T PRR STUDIES ON TRIFLUOPERAZINE-CALMODULIN INTERACTION<br>钙调蛋白与拮抗剂TFP相互作用的水质子弛豫速率研究
%A Ou Yaohua
%A Hu Baocheng
%A Zhou Xin
%A <br>区耀华
%A 胡宝成
%A 周昕
%J 生物物理学报
%D 1988
%I 
%X Trifluoperazine (TFP) is an antipsychotic agent which interacts with Calmodulin (CaM) and is a potent antagonist of CaM.The change of water proton relaxation rates (PRR) by TFP bound to CaMis examined.By measuring T-11p for the system TFP-CaM it is possible to obtainthe hydration number of metal ions bound to the binding sites of CaM.As the result of our experiment, when the ratio of TFP:CaM is not larger than 2,the TFP bound to CaM does not change the metal ion coordination sphere. However, the additional TFP reduces the affinity of CaM to metal ions.The third TFP is believed to be bound to the CaM molecule in a different way.This conclusion is supported by the CD spectra of TFP-CaM determined in the far-UV and near-UV region.
%K Calmodulin
%K Antagonist TFP
%K Water proton relaxation rate<br>钙调蛋白
%K 拮抗剂
%K TFP
%K 水质子弛豫速率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=BAE32571E42E8F0D4AD1178C9877DA2B&yid=0702FE8EC3581E51&vid=E158A972A605785F&iid=38B194292C032A66&sid=0F7768518993EDDE&eid=96A53C367B5173D7&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0