%0 Journal Article
%T Detection of P53 and K-ras Gene Mutations in Lung Cancer with Oligonucleotide Chip<br>应用寡核苷酸微阵列检测肺癌样品中的P53和K-ras基因点突变
%A Jun-Bo Liu
%A Ji-Pan Xie
%A Zong-Liang Zou
%A Lin-Jie Chen
%A Long-Yun Li
%A Sheng-Qi Wang
%A <br>刘军波
%A 解纪攀
%A 邹宗亮
%A 陈琳
%A 李龙芸
%A 王升启
%J 生物工程学报
%D 2002
%I 
%X Different factors including hybridization solution components, hybridization temperature, and the concentration and proportion of the labelled primer, which affected the sensitivity and specificity of single mutation identification, were exploited. Asymmetric PCR increased the hybridization sensitivity, and the asymmetric multi-PCR did not affect the specificity, while the sensitivity was improved a little. Among 30 lung cancer samples detected with the oligonucleotide microarray, 12 was found P53 gene mutations and 5 had K-ras gene mutations. The P53 gene mutations identified by the oligonucleotide microarray was proved 80% same as the sequencing results. The obvious statistical relations of K-ras and P53 gene mutations with tumor type, tumor stage and smoking were not obtained because of less samples and mutation sites.
%K oligonucleotide microarray
%K lung cancer
%K P53
%K K-ras
%K point mutation<br>寡核苷酸微阵列
%K 检测
%K 肺癌样品
%K P53
%K K－ras基因点突变
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8FFE4F26272D45A6&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=C66DE7562B0326E2&eid=9B95A71E6639C039&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=12