%0 Journal Article
%T THE INHIBITIVE EFFECT OF NITRIC OXIDE ON HIPPOCAMPAL NEURONS EXCITATION<br>一氧化氮抑制海马神经元兴奋的机制研究
%A CHEN Xiao-chun
%A SHENG Chao
%A ZHENG Xiao-xiang
%A <br>陈啸春
%A 盛超
%A 郑筱祥
%J 生物物理学报
%D 2001
%I 
%X The inhibitive effect of nitric oxide(NO) on cultured rat hippocampal CA1 neurons excitation stimulated by penicillin G(PG) was investigated. A slow and late type of intracellular NO production induced by PG(1000 IU/ml) was disclosed when monitored with laser scanning confocal microscopy (LSCM). Pretreatment of L-NNA(0-10μmol/L), an NO synthase inhibitor, could dose-dependently inhibit the NO production and increase the intercellular level glutamate(10min after PG stimulation) as well. L-NNA(0-10μmol/L) also tended to increase the immunoreactive methionine enkephalin(ir-M-ENK) level while the immunoreactive dynorphin B(ir-DYN-B) level was remained unchanged. Finally, β-FNA (100μmol/L, an M-ENK receptor inhibitor) but not nor-BIN(100μmol/L, a DYN receptor inhibitor) could inhibit the increase of intercellular level glutamate caused by L-NNA. Together, the following conclusion was made: NO induced by PG (1000 IU/ml) stimulation inhibit further neuron excitation by inhibit the level of M-ENK.
%K Nitric oxide
%K Glutamate
%K Enkephalin
%K Hippocampal Neuron
%K Epilepsy<br>一氧化氮
%K 谷氨酸
%K 脑啡肽
%K 海马神经元
%K 癫痫
%K 兴奋抑制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=9B37AD6BF63F0797&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=38B194292C032A66&sid=8F2250DA83AF77B8&eid=DA4893B5F9885621&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=15