%0 Journal Article
%T Cloning of Staphylococcal Enterotoxin B Gene and Its Highly Expression in Escherichia coli<br>金黄色葡萄球菌肠毒素B基因的克隆和在大肠杆菌中高效表达
%A YANG Li-Quan WU Wen-Fang SHI Cheng-Bo LU An-Guo FENG Jia-Xun
%A BAI Xue-Liang
%A <br>杨立泉
%A 吴文芳
%A 时成波
%A 吕安国
%A 冯家勋
%A 柏学亮
%J 生物工程学报
%D 2002
%I 
%X An about 700 bp DNA fragment was amplified from genome DNA of S. aureus TSTw by PCR. This fragment was cloned into pGEM-7Zf(+) and the recombinant plasmid was transformed into E. coli DH5 alpha. The sequencing result of the recombinant plasmid demonstrated that it contains seb gene with 717 bp (without signal encoding region of 81 bp) which has the same nucleotide sequence as described in literature. The seb gene was cloned into expression vector 7ZTS and was transformed into E. coli JM109 (DE3). The expression level of SEB was as high as 33.3% of the cell total proteins.
%K SEB
%K gene cloning and expression
%K superantigen<br>SEB，
%K 基因克隆和表达，
%K 超抗原
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D8B948E8F118488F&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=0A8675156EB60B87&eid=1D5555D0B4345CA8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=9