%0 Journal Article
%T CIRCULAR DICHROISM AND THERMAL DENATURATION STUDIES OF CHROMATIN AND DNA EROM BRDUTREATED EMBRYONIC CHICKEN NEURAL RETINA<br>5-溴脱氧尿嘧啶核苷对鸡胚神经视纲膜染色质、DNA的园二色性光谱与热变性的影响
%A 付络宁
%A 李公岫
%J 生物物理学报
%D 1986
%I 
%X o-B r o m o - 2' - deoxyuridiue (Brdu), an analogue of thymidine, is used to prob gene regulation during the histogenesis and biochemical differentation in chick embryonic neural retina.After short treatment with Brdu, the histogenesis of 6-day retina was inhibited irreversibly. These effects of inhibition were. however, reversible in 7-day retina and absent in 9 - day retina.The study of CD spectra of DNA from 6, 7 and 9 - day retina showed that Brdu treatment caused a blue shift and an increased positive ellipticity in the 260-280 nm region and an increased negative ellipticity in 290-300 nm region in CD spectra. When the same observation wa; made in the chromatir. these changes in CD spectra were obvious in 6 and 7 day retina, but barely detectable in 9 - day retina. Analysis of thermal stability of Brdu- treated DNA also revealed that Brdu treatment causes an increase in melting temperature and hyperchromic effect in DNA from 6, 7 and 9 - day retina. Again, these effects were apparent only for 6 and 7-day retina.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=B229EDD24E0C44C54199C49CD4AE359E&yid=4E65715CCF57055A&vid=0B39A22176CE99FB&iid=E158A972A605785F&sid=88D36036CFF69B3C&eid=407C905D8F0449C4&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0