%0 Journal Article
%T Effect of Trypsin on the Rat Keratinocyte Separation and Subculture<br>胰酶对皮肤角质细胞分离和传代的影响
%A OUYANG An-Li
%A ZHOU Yan
%A HUA Ping
%A TAN Wen-Song
%A <br>欧阳安力
%A 周燕
%A 华平
%A 谭文松
%J 生物工程学报
%D 2002
%I 
%X The effect of trypsin on the separation an subculture of the keratinocytes was investigated in this work.It was found that when 0 25% trypsin was employed for 5 minutes to separate keratinocytes,the number of active keratinocytes and the cells capable of forming colony were higher than those of other experimental conditions.The maximum attached ratio of primary keratinocytes was obtained when skin tissues were treated at 0 05% concentration of trypsin.With the increase of the trypsin concentrations,the attached ratio,attachment rate constant,and colony forming efficiency were all increased.Thus,0 25% concentration of trypsin was recommended for separating and subculturing the keratinocytes.
%K keratinocyte
%K trypsin
%K attached ratio
%K colony forming efficiency<br>角质细胞
%K 胰酶
%K 贴壁率
%K 克隆形成率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5389242EE6FE6B1D&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=6AC2A205FBB0EF23&eid=95D537AC89B28832&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=7&reference_num=13