%0 Journal Article
%T The Effect of Antioxidants on the in vitro Life-span of Keratinocyte<br>抗氧化剂对皮肤角质细胞体外寿命的影响
%A ZHOU Yan
%A OUYANG An-Li
%A HUA Ping
%A TAN Wen-Song
%A <br>周燕
%A 欧阳安力
%A 华平
%A 谭文松
%J 生物工程学报
%D 2002
%I 
%X The effect of antioxidants on the in vitro life span of mouse keratinocytes was investigated in this work. It was found that the life span of the keratinocytes cultured in the medium supplemented with antioxidants was extended significantly. The most beneficial antioxidant used in this work was the mercaptoethanol, followed by the catalase and SOD. However, the growth rates of keratinocytes in vitro under all the experimental conditions still declined with the culture time. It was also found that the antioxidants added in the medium were also helpful to enhance the keratinocyte colony formation. In addition, the aging kinetics of the mouse epidermal keratinocytes in vitro were analyzed, and finally the aging rate constants corresponding to antioxidants used were calculated.
%K keratinocyte
%K life span
%K antioxidant
%K senescence<br>角质细胞，
%K 抗氧化剂，
%K 体外寿命
%K 细胞衰老
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D9F30192342EDE1C&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=039DCCB9394D9766&eid=06F643376BC2509E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=11