%0 Journal Article
%T Modification of L-asparaginase with Colominic Acid and the New Characteristics of the Modified Enzyme<br>多聚唾液酸对L-天冬酰胺酶的修饰及修饰酶特性研究
%A WANG Ying-Da
%A GUO Li
%A QIAN Shi-Jun
%A MENG Guang-Zhen
%A Zhang Shu-Zheng
%A <br>王颖达
%A 郭丽
%A 钱世钧
%A 孟广震
%A 张树政
%J 生物工程学报
%D 2000
%I 
%X The colominlc acid was covalently coupled to L-asparaginase molecule by reductive amination. Depending on the molar ratios of colominic acid-asparaginase (301,501 and 1001),a modified enzyme molecule contained 4.7,7.2 and 12 colominic acid molecule,they retained 58%,56% and 33.2% of the initial asparaginase activity,respectively. In comparison with the native enzyme, modified enzyme had lower immunogenicity and antigenicity,longer half-life time (in uitro ),more resistance ability to trypsin proteolysis, and similar Km value for L-asparagine.
%K L-asparaginase
%K colominic acid
%K immunogenicity
%K antigenicity
%K proteolysis<br>L－天冬酰胺酶
%K 多聚唾液酸
%K 免疫原性
%K 淋巴系肿瘤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=39F6C14C776969FC&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=E158A972A605785F&sid=5A751AE9FA58A3FB&eid=F3A3627BFEF439C1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=12