%0 Journal Article
%T Expression and Characterization of Two Kinds of Recombinant Snake Neurotoxins<br>蛇神经毒素的表达和鉴定
%A QIAN You-Cun
%A SHEN Yan
%A FAN Chun-Yang
%A HU Tai-Shan
%A YANG Sheng-Li
%A GONG Yi
%A <br>钱友存
%A 沈雁
%A 范春阳
%A 胡太山
%A 杨胜利
%A 龚毅
%J 生物工程学报
%D 2000
%I 
%X The cDNA encoding the precursor of cobrotoxin was cloned from the venom gland of the Chinese continental cobra ( Naja naja atra ) by RT PCR.Its deduced amino acid sequence analysis showed that the mature protein was identical to that identified from the Taiwan cobra ( Naja naja atra ) by protein sequencing technique.The cDNA encoding the mature protein was then subcloned into the expression vector pMAL P 2.The gene of CM11,which was formed by ligation of the fragments of the synthetic oligonucleotides,was also cloned into the expression vector pMAL P 2.After induction of IPTG,both of the neurotoxins were overexpressed as soluble fusion proteins which were confirmed by SDS PAGE and western blotting.The expressed fusion proteins was purified by sepharose 6B amylose affinity chromatography and DEAE sepharose FF chromatography.Both of the recombinant proteins achieved after digestion by factor Xa showed the in vivo toxicity.
%K Neurotoxin
%K fusion expression
%K genetic engineering<br>神经毒素，
%K 融合表达，
%K 基因工程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF8CD2CFB6A1BF36&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=7737D2F848706113&eid=0C3F9E980968AF79&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=10