%0 Journal Article
%T Construction of Yeast Fusants of Directly Transform Starch into Ethanol<br>构建直接发酵淀粉产生酒精的酵母融合菌株的研究
%A PANG Xiao
%A |Yan
%A WANG Ji
%A |Ying
%A ZHAO Feng
%A |Sheng
%A <br>庞小燕
%A 王吉瑛
%A 赵凤生
%J 生物工程学报
%D 2001
%I 
%X To obtain strains that are able to efficiently produce ethanol directly from starchy material, we have used the protoplast fusion technique to construct hybrids between Saccharomyces cerevisiae and Candida tropicalis. The isolation of fusants used a selective system by a irreversible biochemical inhibitor-Iodoacetic Acid(ID), and the fusion frequency was 9.2 x 10(-6). The cell size and DNA content of the fusants were determined. Through testing some productive properties such as specific growth rate, saccharification ability, ethanol tolerance and the activities of alpha-amylase, saccharogen amylase, we selected two fusants--F-1 and F-5. They were more superior and their ethanol production came up to 8.8% and 11.5%.
%K Saccharomyces cerevisiae\%
%K \?ndida tropicalis\%
%K protoplast
%K fusion
%K alcoholic fermentation<br>酒精酵母，热带假丝酵母，原生质体融合，酒精发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5F9B4BA0AB37F8F0&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=31611641D4BB139F&eid=954CE65414DD94CA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=28&reference_num=13