%0 Journal Article
%T Asymmetric Biosynthesis of Chiral Styrene Oxide<br>手性苯基环氧乙烷的生物不对称合成
%A WU Jin
%A CHENG Shu-Hua
%A SHA Qian
%A YANG Liu
%A SUN Wan-Ru
%A <br>吴襟
%A 程树华
%A 沙倩
%A 杨柳
%A 孙万儒
%J 生物工程学报
%D 2000
%I 
%X Used styrene as the sole carbon and energy source, 12 strains of aerobic bacterial and two strains of fungi were screened from a series soil samples. These strains were able to convert styrene to styrene oxide. HPLC and chiral GC method were used for determination the substrate, product and e.e value of culture broth, There were obvious effect on the biomass and epoxidase activity under various conditions in the fermentation. Styrene epoxidation of the strain PS-1206 was studied in different reaction time. 35% of conversion yield and 80% e.e value of (R)-styrene oxide could been obtained at the optimum conditions.
%K Epoxidase
%K styrene
%K chiral styrene oxide
%K asymmetric biosynthesis<br>环氧化酶，苯乙烯，手性苯基环氧乙烷，不对称生物合成
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D411A9B4D9175AD5&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=94C357A881DFC066&sid=821800203AD09E7B&eid=039DCCB9394D9766&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=9