%0 Journal Article
%T Expression and Purification of Murine Interleukin 18 in Escherichia coli and Its Antitumor Effects<br>小鼠白细胞介素18在大肠杆菌中的表达、纯化及抗肿瘤作用研究
%A ZHOU Hu
%A ZHAO Hui
%A |Ren
%A PEI Dong
%A |Sheng
%A LU Liang
%A Hu Shu
%A |Qun
%A <br>周虎
%A 赵惠仁
%A 裴冬生
%A 陆梁
%A 胡书群
%J 生物工程学报
%D 2001
%I 
%X Total RNA was extracted from murine hepatocytes, and the cDNA of interleukin 18(IL-18) was amplified by RT-PCR. The cDNA was introduced into the expression vector pJW2 and sequenced. Under heat induction, the recombinant murine IL-18(rmIL-18) was expressed in inclusion bodies in E. coli with the yield accounting for 18% of total bacteria proteins. The inclusion bodies were dissolved with 5 mol/L urea, and rmIL-18 was purified using Sephadex G-100 column chromatography. In the presence of 0.5 mg/L Con A, the purified rmIL-18 showed dose-dependent IFN-gamma-inducing activity in murine splenocytes. The purified rmIL-18 exhibited significant antitumor effects in Kunming mice challenged intraperitoneally (i.p.) with H22 hepatocarcinoma when administered 10 micrograms rmIL-18 i.p. on days 1, 4 after challenge, and the mice survived resisted the rechallenged with H22 cells.
%K murine interleukin 18
%K cloning
%K expression
%K purification
%K renaturation
%K H
%K 22
%K hepatocarcinoma<br>小鼠白细胞介素18
%K 克隆
%K 表达
%K 纯化
%K 复性
%K H22肝癌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=82557E800C7B4A0C&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=E5D85F291CED2DA6&eid=385E3C2062167B88&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=18