%0 Journal Article
%T THE STUDIES ON THE RED BLOOD CELL MEMBRANE AFFECTED BY MERCURIDES<br>汞化合物对红细胞膜作用的研究
%A 程极济
%A 周培林
%A 陈水月
%J 生物物理学报
%D 1986
%I 
%X This paper reports the effects of mercurides on the red blood cell membrane including the changes of fluorescence and phosphorescence spectra of membrane protein; the fluorescence polarization of DPH labelled on membrane; the bindings of membrane with ANS and their relationships with the hemolysis of RBC affected by mercurides.The intensity of fluorescence of the membrane protein decreased with the increase of the concentration of mercurides. The quenching effect of the mercurides in 5p7.5 buffer solution is HgCl2> Hg(AC)2 PCMB. The quenching effect is two phases process in both 5p7.5 and PBS buffer solutions, but the slow phase of the quenching curve in PBS buffer solution changes very little. The fluorescence quenching effect of mercurides is due to the formation of non-fluorescent complex of R-Trp-Hg+ and the energy transfer from the Trp to the complex of R-S-Hg+. The intensity of phosphorescence of the membrane protein also decreased with the increase of the concentrtaion of HgCl2, but the ratio of P/F increased. As the concentration of HgCl2 and PCMB increase, the fluorescence polarization of DPH labelled on the ghost increased. The increase of polarization means the decrease of membrane fluidity which it may cause the hemolysis of the red blood cells. The dindings of ANS on the ghost protein increased with increase of mercurides, this result also coincides with the hemolvsis of RBC.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=6ACBC0D58AACC945A98E8D6B4158FA83&yid=4E65715CCF57055A&vid=0B39A22176CE99FB&iid=CA4FD0336C81A37A&sid=ECE8E54D6034F642&eid=771152D1ADC1C0EB&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=1&reference_num=0