%0 Journal Article
%T Cloning and Expressing of An Anti-CD5 Single Chain Antibody<br>抗CD5单链抗体基因克隆和表达
%A WANG Fei
%A MA Qing
%A |Jun
%A <br>王菲
%A 马清钧
%J 生物工程学报
%D 2001
%I 
%X Novel anti\|CD5 single\|chain fragment of variable domain (ScFv) was cloned and expressed.Anti\|CD5 ScFv was constructed with cDNA fragments of heavy and light variable regions (V H and V L) which were reverse\|translated from poly (A) mRNA of hybridoma cells producing anti\|CD5 McAb.By phage displaying,ScFv in form of ScFv\|g3p fusion protein was panned based on its binding capacity to the CD5 antigens on the cell surface of Molt\|4 cells.Assayed by the cell\|ELISA,4 clones were found to have high affinity to CD5 antigen.DNA sequencing confirmed that the V H fragment is 339 base pairs,the V L 300bp,and the ScFv belongs to the mice gene family.In \%E.coli\% HB2151 the soluble ScFv\|CD5 was expressed mainly in the periplasm.
%K single\|chain fragment of variable domain
%K CD5\|antigen
%K monoclonal antibody<br>单链抗体，
%K CD5抗原，
%K 克隆，
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5FAC9433674EB71B&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=6DE26652A1045643&eid=03A030BB0C519C60&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=20