%0 Journal Article
%T Expression of Human Interleukin-11 in Cell Culture and Larvae of Silkworm<br>人白细胞介素-11基因在家蚕培养细胞和虫体内的表达
%A GUO Xi-Jie
%A JIN Yong-Feng
%A YANG Ming-Guan
%A ZHANG Yao-Zhou
%A <br>郭锡杰
%A 金勇丰
%A 杨明观
%A 张耀洲
%J 生物工程学报
%D 2002
%I 
%X A recombinant transfer vector, pBacIL-11, containing hIL-11 cDNA of 546 nucleotides lacking leader sequence was constructed and co-transfected into BmN cells with linearized BmBacPAK(modified BmNPV) DNA for construction of a recombinant baculovirus carrying the hIL-11 gene. Southern hybridization analysis suggested that the recombinant baculovirus DNA contained hIL-11 cDNA fragment.RNA dot blotting demonstrated that the hIL-11 gene was transcribed. The recombinant baculovirus has a strong infectivity to BmN cell line and to silkworm larvae and pupae. Specific hIL-11 bands were detected from all the samples of cell extract, culture supernatant, haemolymph of larvae and pupae by SDS-PAGE analysis. Biological activity of the expressed product was determined with IL-11 dependent B 9-11 cell line and by MTT colorimetric assay, which indicated that biolo gically active rhIL-11 protein was overexpressed in BmN cell line and in silkworm larvae and pupae.
%K human interleukin-11
%K recombinant baculovirus
%K cell culture and silkworm
%K expression<br>人白细胞介素－11基因
%K 家蚕培养细胞
%K 虫体内
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=174817335BA41FBC&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=340AC2BF8E7AB4FD&eid=339D79302DF62549&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15