%0 Journal Article
%T Construction of Luciferase Reporter Plasmid Which is Under the Control of Dioxin-responsive Enhancers<br>二恶英反应增强子调控的虫荧光素酶报告基因质粒的构建
%A Z R Zhang
%A S Q Xu
%A Y K Zhou
%A S Ren
%A B Lü
%A <br>张志仁
%A 徐顺清
%A 周宜开
%A 任恕
%A 吕斌
%J 生物工程学报
%D 2001
%I 
%X To improve the bio\|detection of dioxin\|type chemicals,we constructed a plasmid containing reporter under the control of dioxin\|responsive enhancer.After connected the enhancer from the plasmid pHAV with the MMTV promoter of the plasmid pCatM,the connected segment was linked with the luciferase vector.Then the recombinant plasmid was transfected to the HepG2 human hepatoma cell line,and the luciferase activity was induced by 2,3,7,8\|TCDD.The result suggested that expression of the recombinant luciferase was controlled by the dioxin\|responsive enhancer,and there were a linear relationship between the activity of luciferase and certain range of 2,3,7,8\|TCDD concentration.It is promising that the transfected cell line could be used for rapid screen and semi quantitation of dioxin\|type chemicals,and a standard and routine method for the detection of dioxin\|type chemicals by this recombinant plasmid is being under way.
%K dioxin\|type chemicals
%K luciferase
%K reporter gene
%K dioxin\|responsive enhancer<br>二恶英类化学物质，
%K 虫荧光素酶，
%K 报告基因，
%K 二恶英反应增强子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C29A1DE1FFAC4B7F&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=C5F8B8CB20F1B3D8&eid=0584DB487B4581F4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=10