%0 Journal Article
%T Construction and Expression of Human Micro Plasminogen Gene in Escherichia coli<br>人微小纤溶酶原基因的克隆和表达
%A Shen Junqing
%A Song Houyan
%A <br>沈俊卿
%A 宋后燕
%J 生物工程学报
%D 1997
%I 
%X Human mPlg was amplified by PCR, using pBS S1 cDNA encoding human plasmingen as a templete. The mPlg gene was cloned into expression vector and transfected into E.coli JF1125. SDS PAGE analysis revealed that expression product was Mr.29 and about 24% of total bacteria protein and formed inclusion body. After refoding by Air Oxidation and Cysteine Reoxidation, the fibrinolysis activity of r mPlg was shown. Effects of protein concentration and refolding time on renaturation efficiency were discuss.
%K Human microplasminogen
%K cloning
%K expression
%K refolding<br>人微小纤溶酶原，克隆．表达，体外复性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5E8F92F1659C495788F1C57EA74C2D34&yid=5370399DC954B911&vid=FC0714F8D2EB605D&iid=CA4FD0336C81A37A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0